Coding
P1003 5*

Part:BBa_K2725012:Design

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)


P1003 Cassette with 5 amber stop codons


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Serine -> Amber substitutions were chosen to be spread evenly throughout the coding sequence.


Source

Edits made to BBa_K914009 sequence from Paris Bettencourt 2012 project.

References